http://ukrbiochemjournal.org The official site Thu, 22 Jun 2017 08:38:00 +0000 en-US hourly 1 http://ukrbiochemjournal.org/2017/05/theses-of-reports-of-the-25th-annual-conference-modern-aspects-of-biochemistry-and-biotechnology-and-2nd-conference-for-young-scientists-of-the-division-of-biochemistry-physiology-a.html http://ukrbiochemjournal.org/2017/05/theses-of-reports-of-the-25th-annual-conference-modern-aspects-of-biochemistry-and-biotechnology-and-2nd-conference-for-young-scientists-of-the-division-of-biochemistry-physiology-a.html#comments Wed, 24 May 2017 08:53:53 +0000 http://ukrbiochemjournal.org/?p=4270 Organizers: Palladin Institute of Biochemistry of NAS of Ukraine Ukrainian Biochemical Society Council of Young Scientists of the Division of Biochemistry, Physiology and Molecular Biology National Academy of Sciences of Ukraine Sections: 1. Biomaterials 2. Bioactive compounds 3. Cancerogenesis 4. Gene expression 5. Metabolites and correction of metabolic processes 6. Proteomics and protein functions 7. Molecular basis of physiological functions ]]> http://ukrbiochemjournal.org/2017/05/theses-of-reports-of-the-25th-annual-conference-modern-aspects-of-biochemistry-and-biotechnology-and-2nd-conference-for-young-scientists-of-the-division-of-biochemistry-physiology-a.html/feed 0 http://ukrbiochemjournal.org/2017/05/development-of-the-method-for-microbiological-purity-testing-of-recombinant-human-interleukin-7-based-product.html http://ukrbiochemjournal.org/2017/05/development-of-the-method-for-microbiological-purity-testing-of-recombinant-human-interleukin-7-based-product.html#comments Wed, 24 May 2017 08:47:05 +0000 http://ukrbiochemjournal.org/?p=4267 O. Yu. Galkin 1 , T. M. Lutsenko 1,2 , Yu. V. Gorshunov 1 , V. V. Motronenko 1 1 National Technical University of Ukraine “Ihor Sikorskii Kyiv Polytechnic Institute”, Ukraine; 2 UA “PRO-PHARMA” LLC, Kyiv, Ukraine; e-mail: alexfbt@gmail.com The goal of the work was justification of standardization parameters of the product based on recombinant human IL-7 by “microbiological purity” parameter and development of its measurement method with consideration of the requirements of guideline documents regarding quality of medicinal products. Suitability assessment of microbiological purity testing procedures was performed. It has been established that the studied product in standard testing methods possessed antimicrobial activity against test microorganisms Basillus subtilis ATCC 6633 (on soybean casein digest agar and Sabouraud dextrose agar containing an antibiotic), Candida albicans АТСС 10231 (on soybean casein digest agar and Sabouraud dextrose agar containing an antibiotic), and possessed no antimicrobial activity against test microorganisms Staphylococcus aureus ATCC 6538 (on soybean casein digest agar) and Pseudomonas aeruginosa ATCC 9027 (on soybean casein digest agar). The efficacy of lecithin and polysorbate as substances eliminating antibacterial effect in combination with 50-fold dilution of the test sample has been suggested and proven for the purpose of antimicrobial activity neutralization. The developed total aerobic microbial count measurement procedure for the test product conforms to acceptance criteria provided by SPU (2.6.12), and can be used for its microbiological purity control. ]]> http://ukrbiochemjournal.org/2017/05/development-of-the-method-for-microbiological-purity-testing-of-recombinant-human-interleukin-7-based-product.html/feed 0 http://ukrbiochemjournal.org/2017/05/gcmc-analysis-of-constituent-oil-from-tremella-fuciformis-white-jelly-mushrooms-from-nigeria.html http://ukrbiochemjournal.org/2017/05/gcmc-analysis-of-constituent-oil-from-tremella-fuciformis-white-jelly-mushrooms-from-nigeria.html#comments Wed, 24 May 2017 08:42:52 +0000 http://ukrbiochemjournal.org/?p=4264 Reginald C. Ohiri Department of Biochemistry, Faculty of science, University of Port Harcourt; East-West Road, Choba, Rivers State, Nigeria; e-mail: raycohiri@yahoo.com, reginald.ohiri@uniport.edu.ng The use of mushrooms for the treatment of diseases can be traced to Paleolithic era. One mushroom that has shown potent medicinal properties both by oral consumption and topical application is the Tremella fuciformis . GC/MS analyses of T. fuciformis oil obtained by hydro-distillation showed four predominant compounds. 9,19-Cyclolanost-24-en-3-ol, (3.beta.)- was the most predominant with R T of 20.766 and 24.672 min and a percentage total of 32.681 followed by 7-Isopropenyl-1,4a-dimethyl-4,4a,5 ,6,7,8-hexahydro-3H-naphthalen-2-one with R T of 22.425 min and a percentage total of 21.334, lupeol had R T of 22.825 and 23.230 min and a percentage total of 18.531, while lanosterol had R T of 21.079 and 23.034 min and a percentage total of 17.845. These four compounds represent 90.391% of the total constituent compounds in T. fuciformis oil. The therapeutic properties of two predominant compounds (lanosterol and lupeol) of this mushroom confirm its use for the treatment of both dermatological and ophthalmological related diseases by traditional medical practitioners. ]]> http://ukrbiochemjournal.org/2017/05/gcmc-analysis-of-constituent-oil-from-tremella-fuciformis-white-jelly-mushrooms-from-nigeria.html/feed 0 http://ukrbiochemjournal.org/2017/05/corvitin-improves-metallothionein-and-glial-fibrillary-acidic-protein-levels-in-rat-brain-affected-by-pituitrin-izadrin.html http://ukrbiochemjournal.org/2017/05/corvitin-improves-metallothionein-and-glial-fibrillary-acidic-protein-levels-in-rat-brain-affected-by-pituitrin-izadrin.html#comments Wed, 24 May 2017 08:38:54 +0000 http://ukrbiochemjournal.org/?p=4260 H. N. Shiyntum 1 , O. O. Dovban 1 , Y. P. Kovalchuk 1 , T. Ya. Yaroshenko 2 , G. A. Ushakova 1 1 Oles Honchar Dnipro National University, Ukraine; 2 I. Horbachevsky Ternopil State Medical University, Ukraine; e-mail: hnkafor@yahoo.com In this research, we investigated the effect of pituitrin-izadrin induced injury on the levels of metallothionein (MT) and soluble and filament forms of glial fibrillary acidic protein (GFAP) in the hippocampus, cerebellum, thalamus, and the cerebral cortex, and examined the effect of corvitin on the brain under the noted changes. Our results showed oppositely directed changes – a decrease in the level of MT and an increase in GFAP in the rat brain, with a tendency to astrogliosis development, under the influence of systemic deficiencies in myocardial function. The use of corvitin at a dose of 42 mg/kg for five days after a cardiac attack caused by pituitary-izadrin leads to recovery in the balance of the studied proteins. ]]> http://ukrbiochemjournal.org/2017/05/corvitin-improves-metallothionein-and-glial-fibrillary-acidic-protein-levels-in-rat-brain-affected-by-pituitrin-izadrin.html/feed 0 http://ukrbiochemjournal.org/2017/05/imunobiochemical-changes-and-their-diagnostic-value-in-the-blood-in-patients-with-acute-ischemic-stroke.html http://ukrbiochemjournal.org/2017/05/imunobiochemical-changes-and-their-diagnostic-value-in-the-blood-in-patients-with-acute-ischemic-stroke.html#comments Wed, 24 May 2017 08:35:52 +0000 http://ukrbiochemjournal.org/?p=4257 O. Ja. Mykhalojko, І. Ja. Mykhalojko Ivano-Frankivsk National Medical University, Ukraine; e-mail: myhalojko@i.ua The aim was to determine the alterations in levels of vasculair endothelial growth factor (VEGF), homocysteine (HC) and lipid profile in patients with acute ischemic stroke (AIS), to establish the relationship between them. A clinical and neurological examination of 120 patients aged 56 to 75 years with AIS was done. Diagnosis was established taking into account results of spiral computer and magnetic resonance tomography. The control group included 20 people with no signs of acute cerebrovascular and severe somatic pathology aged 59.35±1.8 years. Assessment of disability was performed at admission and during the treatment using the scale NIHSS. In all relevant patients in 1-2 days after stroke, the concentration of HC and VEGF levels was determined by ELISA. Blood lipid profile was determined by enzymatic cоlorimetric method. The study found that increasing concentration of plasma HC is associated with intensity of hyperlipidemia, and hyperhomocysteinemia severity prevails over the changes in the lipid profile components. During AIS a significant increase in the level of VEGF in the blood was observed, indicating the presence of vascular accident and activation of angiogenesis. Severity of vascular endothelial lesions is associated with the degree of increasing concentration VEGF that appears most significant in patients with severe AIS. ]]> http://ukrbiochemjournal.org/2017/05/imunobiochemical-changes-and-their-diagnostic-value-in-the-blood-in-patients-with-acute-ischemic-stroke.html/feed 0 http://ukrbiochemjournal.org/2017/05/the-effect-of-thymic-mesenchymal-stromal-cells-on-arginase-activity-and-nitric-oxide-produced-by-macrophages-of-young-and-aged-mice.html http://ukrbiochemjournal.org/2017/05/the-effect-of-thymic-mesenchymal-stromal-cells-on-arginase-activity-and-nitric-oxide-produced-by-macrophages-of-young-and-aged-mice.html#comments Wed, 24 May 2017 08:32:18 +0000 http://ukrbiochemjournal.org/?p=4254 R. S. Dovgiy 1,2 , I. S. Nikolsky 3 , L. M. Skivka 1 1 Taras Shevchenko National University of Kyiv, Ukraine; 2 Institute of Gerontology, NAMS of Ukraine, Kyiv; 3 State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, Kyiv; e-mail: romandovgiy@gmail.com Mesenchymal stromal cells (MSC) gained much attention due to their therapeutic properties, mediated largely by anti-inflammatory action. We aimed to investigate the capacity of MSC obtained from young mice to modulate arginine metabolism of macrophages from old animals. Bone marrow cells obtained from young and aged mice were cocultivated with MSC in the presence of M-CSF. Nitric oxide production was analyzed in supernatants by Griess reaction, and arginase activity was measured in cell lysates. We have found that arginase activity was significantly lower in macrophages isolated from old mice as compared to young animals ( P ˂ 0.05). Syngeneic MSC addition markedly stimulated arginase activity in macrophages from both young and aged mice ( P ˂ 0.001), with greater effect in old animals. There were no significant differences in nitric oxide level between groups. In summary, there was more pronounced anti-inflammatory shift in macrophage metabolism in aged animals upon cocultivation with MSC. ]]> http://ukrbiochemjournal.org/2017/05/the-effect-of-thymic-mesenchymal-stromal-cells-on-arginase-activity-and-nitric-oxide-produced-by-macrophages-of-young-and-aged-mice.html/feed 0 http://ukrbiochemjournal.org/2017/05/ca2-dependent-regulation-of-the-ca2-concentration-in-the-myometrium-mitochondria-ii-ca2-effects-on-mitochondria-membranes-polarization-and-ca2m.html http://ukrbiochemjournal.org/2017/05/ca2-dependent-regulation-of-the-ca2-concentration-in-the-myometrium-mitochondria-ii-ca2-effects-on-mitochondria-membranes-polarization-and-ca2m.html#comments Wed, 24 May 2017 08:28:00 +0000 http://ukrbiochemjournal.org/?p=4251 L. G. Babich, S. G. ShlykoV, A. M. Kushnarova, S. O. Kosterin Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv; e-mail: babich@biochem.kiev.ua It is known that Ca 2+ accumulation in the mitochondria undergoes complex regulation by Ca 2+ itself. But the mechanisms of such regulation are still discussed. In this paper we have shown that Ca ions directly or indirectly regulate the level of myometrium mitochondria membranes polarization. The additions of 100 µM Ca 2+ were accompanied by depolarization of the mitochondria membranes. The following experiments were designed to study the impact of Ca 2+ on the myometrium mitochondria [Ca 2+ ] m . Isolated myometrium mitochondria were preincubated without or with 10 μM Са 2+ followed by 100 μM Са 2+ addition. Experiments were conducted in three mediums: without ATP and Mg 2+ (0-medium), in the presence of 3 mM Mg 2+ (Mg-medium) and 3 mM Mg 2+ + 3 mM ATP (Mg,ATP-medium). It was shown that the effects of 10 μM Са 2+ addition were different in different mediums, namely in 0- and Mg-medium the [Ca 2+ ] m values increased, whereas in Mg,ATP-medium statistically reliable changes were not registered. Preincubation of mitochondria with 10 μM Са 2+ did not affect the [Ca 2+ ] m value after the addition of 100 μM Са2+. The [Ca 2+ ] m values after 100 μM Са 2+ addition were the same in 0- and Mg,ATP-mediums and somewhat lower in Mg-medium. Preliminary incubation of mitochondria with 10 μM Са 2+ in 0- and Mg-mediums reduced changes of Fluo 4 normalized fluorescence values that were induced by 100 μM Са 2+ additions, but in Mg,ATP-medium such differences were not recorded. It is concluded that Са 2+ exchange in myometrium mitochondria is regulated by the concentration of Ca ions as in the external medium, so in the matrix of mitochondria. The medium composition had a significant impact on the [Са 2+ ] m values in the absence of exogenous cation. It is suggested that light increase of [Са 2+ ] m before the addition of 100 μM Са 2+ may have a positive effect on the functional activity of the mitochondria. ]]> http://ukrbiochemjournal.org/2017/05/ca2-dependent-regulation-of-the-ca2-concentration-in-the-myometrium-mitochondria-ii-ca2-effects-on-mitochondria-membranes-polarization-and-ca2m.html/feed 0 http://ukrbiochemjournal.org/2017/05/biological-effects-of-lithium-fundamental-and-medical-aspects.html http://ukrbiochemjournal.org/2017/05/biological-effects-of-lithium-fundamental-and-medical-aspects.html#comments Wed, 24 May 2017 08:21:15 +0000 http://ukrbiochemjournal.org/?p=4248 A. S. Mikosha, O. I. Kovzun, M. D. Tronko State Institution V. P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, Kyiv; e-mail: asmikosha@gmail.com In this review, the authors present data on the biological effects of lithium in human and animal body and the subsequent manifestations of these effects. Since the lithium ion radius is close to that of magnesium, lithium ion can compete with magnesium as cofactor of certain magnesium-dependent enzymes. Numerous studies have shown lithium to be an inhibitor of glycogen synthase kinase 3. It also inhibits the phosphatases involved in the metabolism of phosphoinositides and phosphoadenylate 3′-nucleotidase. As these enzymes play an important and sometimes crucial role in signaling systems activity, lithium is able to affect the essential physiological, adaptive and pathogenetic processes. ]]> http://ukrbiochemjournal.org/2017/05/biological-effects-of-lithium-fundamental-and-medical-aspects.html/feed 0 http://ukrbiochemjournal.org/2017/04/sterols-biosynthesis-by-soil-streptomycetes.html http://ukrbiochemjournal.org/2017/04/sterols-biosynthesis-by-soil-streptomycetes.html#comments Wed, 26 Apr 2017 08:29:41 +0000 http://ukrbiochemjournal.org/?p=4201 L. O. Biliavska, A. M. Ostapchuk, S. I. Voychuk, G. O. Iutynska Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv; e-mail: bilyuvskal@gmail.com, galyna.iutynska@gmail.com The goal of this work was to study biosynthesis of sterols by soil streptomycetes Streptomyces avermitilis IMV Ac-5015, Streptomyces netropsis IMV Ac-5025 and Streptomyces violaceus IMV Ac-5027 on various media under submerged cultivation and to determine their content in bioformulations, developed on the basis of these metabolites producers. Analysis of sterol derivatives extracted from biomass, supernatant of cultural liquid and biological products were performed by GC/MS. The biomass of streptomycetes contained sterols in significantly higher amounts than in the cultural supernatants, and their spectrum and quantitative ratio were different. Squalene – sterol precursor – was found in the biomass in quantities producers 6.2-43.3 μg/g, when streptomycetes grown in synthetic and 8.2-212.1 μg/g in organic media. The biomass of S. avermitilis IMV Ac-5015 was distinguished by domination of 24-epibrassinolide, the content of which was the highest among the tested strains and reached 268.2 μg/g in organic and 345 μg/g in synthetic media. S. avermitilis IMV Ac-5015 did not synthesize sitosterol and stigmasterol that is important in respect of nematicidal properties of the strain. The biomass of S. netropsis IMV Ac-5025 and S. violaceus IMV Ac-5027 grown on organic medium contained 2.3-6.8-fold higher amount of sitosterol and 1.5-3-fold higher amount of stigmasterol compared to synthetic medium. Ergosterol prevailed in sterol spectrum of S. netropsis IMV Ac-5025 and S. violaceus IMV Ac-5027. The highest total content of sterols (9.5 mg/l) was found in Avercom (producer – S. avermitilis ІМV Ас-5015). The use of exogenous sterols of microbial origin is important for regulation of their ratio in plants and for increasing resistance to pathogens and phytonematodes. ]]> http://ukrbiochemjournal.org/2017/04/sterols-biosynthesis-by-soil-streptomycetes.html/feed 0 http://ukrbiochemjournal.org/2017/04/scientific-and-practical-activity-of-the-department-of-neurochemistry-of-the-palladin-institute-of-biochemistry-of-nas-of-ukraine.html http://ukrbiochemjournal.org/2017/04/scientific-and-practical-activity-of-the-department-of-neurochemistry-of-the-palladin-institute-of-biochemistry-of-nas-of-ukraine.html#comments Mon, 24 Apr 2017 14:16:39 +0000 http://ukrbiochemjournal.org/?p=4198 V. M. Danilova, R. P. Vynogradova, G. G. Lugovska Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv; e-mail: valdan@biochem.kiev.ua The article analyzes the scientific and practical activity of the Department of Neurochemistry (formerly called the Department of Biochemistry of the nervous system) of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its development. The most important achievements in decoding the biochemical fundamentals of physiological functions of the nervous system that can be useful for solving some problems of clinical medicine are presented and discussed. ]]> http://ukrbiochemjournal.org/2017/04/scientific-and-practical-activity-of-the-department-of-neurochemistry-of-the-palladin-institute-of-biochemistry-of-nas-of-ukraine.html/feed 0